Examination of a sample of hafted bifaces from the Jamestown Rediscovery assemblage revealed the function and origin of the stone tools and further detailed site chronology. It also identified a correspondence between the different material types of the hafted bifaces and their breakage frequencies. Most of the locally made quartzite triangular hafted bifaces were broken whereas very few of those made of non-local jasper and dark chert exhibited significant breakage. These findings might indicate differential use and offer insight into intercultural exchange or hostilities at the site of the original James Fort.